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. 2019 Feb 4;10:59. doi: 10.3389/fphar.2019.00059

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Copyright © 2019 Palikhe, Ohashi, Sakamoto, Hattori, Kawakami, Andoh, Yamazaki and Hattori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of GRK2 siRNA transfection on IFN-β expression in LPS-stimulated MG6 cells. (A) iNOS protein expression before and 15 h after 100 ng/ml LPS application in the presence of IFNAR1 siRNAs (siIFNAR1) or the negative control siRNAs (siCTL). GAPDH served as loading control. Shown are representative Western blots from three independent experiments in which the same results were obtained. (B) IFN-β mRNA expression levels. Following transfection of GRK2 siRNAs (siGRK2) or the negative control siRNAs (siCTL), cells were exposed to 100 ng/ml LPS for 3 h. The mRNA levels were expressed as a fold increase above control normalized GAPDH. (C) Cell culture media were collected 6 h after application of 100 ng/ml LPS, and the concentrations of IFN-β were measured by the ELISA. The results represent the mean ± SEM for three independent experiments. ^∗P < 0.05 and ^∗∗P < 0.01 by t-test.