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. 2019 Feb 4;10:59. doi: 10.3389/fphar.2019.00059

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Copyright © 2019 Palikhe, Ohashi, Sakamoto, Hattori, Kawakami, Andoh, Yamazaki and Hattori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of GRK2 siRNA transfection on TLR9-mediated IRF1 and IFN-β expression in MG6 cells. (A) IFN-β mRNA expression 3 h after 1 μM CpG-ODN or control ODN (CTL-ODN) application in the presence of GRK2 siRNAs (siGRK2), IRF1 siRNAs (siRF1) or the negative control siRNAs (siCTL). The mRNA levels were expressed as a fold increase above control normalized GAPDH and the results represent the mean ± SEM for three independent experiments. ^∗∗P < 0.01 by t-test. (B) Changes in protein expression of IRF1 and GRK2 after 1 μM CpG-ODN challenge in the presence of GRK2 siRNAs (siGRK2) or the negative control siRNAs (siCTL). GAPDH served as loading control. Shown are representative Western blots from three independent experiments in which the same results were obtained.