Table 1.

Proton and carbon NMR chemical shift assignments for BFT and its metabolite.

Position	5β-Hydroxy-Bufotalin		Bufotalin
	δ1H Mult (J in Hz)	δ13C	δ1H Mult (J in Hz)	Δ13C
1	1.16	23.6	1.19	29.6
	1.94		1.91
2	1.38	24.8	1.28	27.9
	1.83		1.59
3	4.20	68.1	4.14	66.7
4	1.56	36.9	1.24	33.3
	2.11		1.76
5	–	73.5	1.59	35.9
6	1.47	35.1	1.46	26.4
	1.72		1.79
7	1.28	21.5	1.32	21.1
	1.47		1.43
8	1.50	39.1	1.49	42.3
9	1.57	41.4	1.56	35.3
10	1.83	40.7	1.84	35.6
11	1.61	28.7	1.54	21.1
	–		1.36
12	1.25	40.5	1.39	40.9
	1.59		1.50
13	–	49.3	–	49.4
14	–	84.4	–	84.4
15	2.85	57.1	1.53	40.4
			2.65
16	5.52	74.4	5.54	73.6
17	2.59	40.8	2.87	57.2
18	0.79	16.4	0.79	16.4
19	0.95	16.7	0.95	23.7
20	–	116.7	–	116.9
21	7.25	151.1	7.25	151.0
22	8.00	149.0	8.03	149.2
23	6.19	113.2	6.19	113.1
24	–	161.9	–	161.9
25	–	170.0	–	170.0
26	1.87	20.9	1.87	20.9

All spectra were recorded on a Bruker ARX-600 spectrometer, in CDCl₃.