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. 2018 Sep 11;7(2):457–473. doi: 10.1016/j.jcmgh.2018.09.002

Figure 8.

Figure 8

Prolonged MDX-enriched diet induces low-grade intestinal inflammation. Wild-type mice were exposed to 5% MDX diluted in drinking water for 10 weeks. (A) Body weight was recorded every 2 weeks until death (day 70). Data were generated using 10–12 mice per group from 3 independent experiments and are expressed as means ± SEM. Differences between groups were compared using the 2-tailed Student t test. (B) Scatter plots showing stool consistency of mice receiving 5% MDX diluted in drinking water for 10 weeks. Data were generated using 10–12 mice per group of 3 independent experiments and expressed as means ± SD. Differences between groups were compared using the 2-tailed Student t test. (C and D) Representative H&E staining of colon sections and histologic score of wild-type mice exposed to drinking water supplemented or not with 5% MDX for 10 weeks. Scale bars: 100 μm. Data were generated using 10–12 mice per group from 3 independent experiments and expressed as means ± SD. Differences between groups were compared using the 2-tailed Student t test (**P ≤ .01). (E) Expression of IL1β, Lcn-2, and Ern2 RNA transcripts in colon tissues taken from mice fed as indicated in panel A. Data were generated using 6–12 mice per group from 3 independent experiments. Each point in the graph indicates the RNA expression of the specific transcript in the colon of a single mouse; horizontal bars indicate median value. Differences between groups were compared using the Mann–Whitney U test (*P ≤ .05). (F) Immunofluorescence analysis of Muc-2 (green) in colon samples isolated from mice fed with or without MDX for 10 weeks. Scale bars: 25 μm. The figure is representative of 3 separate experiments in which similar results were obtained. Rightpanel shows the number of Muc-2–expressing cells per crypt. Data indicate means ± SD of the positive cells counted in 4 different fields per colon section and were generated using 6–7 mice per group from 3 independent experiments. Differences between groups were compared using the 2-tailed Student t test (**P ≤ .01). (G) Fifteen-hour–fasting blood glucose level in mice fed as indicated in panel A. Data were generated using 4–5 mice per group from 2 independent experiments and expressed as means ± SD. Differences between groups were compared using the 2-tailed Student t test (***P ≤ .001). mRNA, messenger RNA.