Fig. 7. Cell loading, stimulation, and collection from a microfluidic chip with transversal delivery of the EGF solution to a ~500 nL cell chamber.

a SKBR3 cell loading on the chip; b Stimulation with EGF solution (10 nM) infused at ~1 µL/min for ~ 3 min; c Empty cell compartment after quick SKBR3 flush-out with ~150–200 µL hypotonic buffer (NH₄HCO₃10 mM/CH₃OH, 95:5 v/v) for off-chip lysis by sonication