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. 2019 Feb 4;10:52. doi: 10.3389/fpls.2019.00052

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Copyright © 2019 Wang, Zhang, Bi, Liu, Xu, Yu, Cao, Zhu, Wu, Zhan, Anis, Yu, Chen, Cheng and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Map-based cloning of es4. (A) Location of es4 on chromosome. (B) A total of 1061 mutant type F₂ individuals were used for fine mapping. (C) Seven ORF were found. (D) ES4 gene structure and CRISPR/Cas9 site of es4-cas9. (E) Mutant site of es4 and CRISPR/Cas9 site of es4-cas9.