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. 2019 Feb 4;10:52. doi: 10.3389/fpls.2019.00052

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Copyright © 2019 Wang, Zhang, Bi, Liu, Xu, Yu, Cao, Zhu, Wu, Zhan, Anis, Yu, Chen, Cheng and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional complementation and CRISPR/Cas9 knock-out of ES4. (A) Plant of ZH8015, es4, and es4-COM at the mature stage. (B) Plant of ZH8015, es4, and es4-cas9 at the mature stage. (C) OsCPK12 relative expression level in ZH8015, es4, es4-COM, and ES4-cas9. (D) Content of Chlorophyll a and b in flag leaf of ZH8015, es4, es4-COM, and ES4-cas9 at the tillering stage. (E) Net photosynthetic rate (P_n) in flag leaf of ZH8015, es4, es4-COM, and ES4-cas9 at 70 dps (mean ± SD, n = 3), Scale bar = 4 cm. Letters in the figure indicate the results of multiple comparison test, a,b,c indicate significant difference on 0.01 level.