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. 2018 Dec 18;294(6):2046–2059. doi: 10.1074/jbc.RA118.004630

Figure 11.

Figure 11.

Presence or absence of INSIG1 did not lead to significant change in cholesterol content in 293T. A, 293T cells were transfected with empty vector, INSIG1, or INSIG1-D205A, and stained with filipin 48 h later. Stained cells were imaged by a fluorescence microscope. Scale bars, 50 μm. B, insig1 knockout 293T cells were stained with filipin. Stained cells were imaged by a fluorescence microscope. Scale bars, 50 μm. C, fluorescence intensity of filipin in stained cells in A was quantified by ImageJ. Shown is mean ± S.D. (error bars) from three independent experiments. D, fluorescence intensity of filipin in stained cells in B was quantified by ImageJ. Shown is mean ± S.D. from three independent experiments.