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. 2018 Dec 18;294(6):2046–2059. doi: 10.1074/jbc.RA118.004630

Figure 2.

Figure 2.

The overexpression of INSIG1 inhibited the production of HIV-1 in 293T and Jurkat cells. A, INSIG1 was overexpressed in HIV-1–producing 293T cells, and the supernatant was harvested to infect TZM-bl cells to test the virus titer by luciferase activity assay. Both Env and VSV-G enveloped virus production in 293T were inhibited significantly by INSIG1 expression. Shown is mean ± S.D. (error bars) from three independent experiments. ***, p < 0.001 compared with the control sample using an independent t test. B, Pr55Gag and Env/VSV-G in the HIV-1–producing cells and p24 in the supernatant in A were detected by Western blotting. Pr55Gag and its cleavage products in cells and p24 in supernatant were significantly reduced by INSIG1. For envelope glycoproteins, both Env and VSV-G were not changed in the INSIG1-overexpressing 293T cells. C, INSIG1 was overexpressed in HIV-1–producing Jurkat cells, and the supernatant was harvested to infect TZM-bl cells to test the virus titer by a luciferase activity assay. Both Env and VSV-G enveloped virus production in Jurkat were inhibited significantly by INSIG1 expression. Mean ± S.D. from three independent experiments. ***, p < 0.001 compared with the control sample using an independent t test. D, Pr55Gag in the HIV-1–producing cells and p24 in the supernatant in C were detected by Western blotting. Pr55Gag and its cleavage products in cells and p24 in supernatant were significantly reduced by INSIG1.