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. 2018 Dec 18;294(6):2046–2059. doi: 10.1074/jbc.RA118.004630

Figure 5.

Figure 5.

Knockout of insig1 in Jurkat increased HIV-1 replication. A, insig1 knockout and control Jurkat cells were infected with equal amounts of Env or VSV-G enveloped pseudovirus. Western blotting detection of infected cells indicated that the protein level of Pr55Gag was increased in insig1 knockout Jurkat cells. B, cell proliferation rate of insig1 knockout Jurkat cells and the control cells. Knockout of insig1 has no obvious effect on the growth of Jurkat cells. The assay was started with 50,000 cells. Data shown are representative of three independent experiments. C, absence of INSIG1 increased the amount of virus released to the medium. insig1 knockout Jurkat cells and the control cells were infected with NL4-3 virus with a titer of 50 pg of p24. Supernatants were harvested at the indicated time, and virus replication was monitored using p24 ELISA. Data shown were representative of three independent experiments.