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. 2018 Dec 6;294(6):2021–2035. doi: 10.1074/jbc.RA118.005978

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© 2019 Yi et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — INCENP interacts with the CSD of HP1α and HP1γ. A, lysates of nocodazole-arrested mitotic HeLa cells transiently expressing VSV-INCENP were subjected to pulldown by GST or the indicated GST fusion proteins followed by anti-VSV immunoblotting and Coomassie Brilliant Blue (CBB) staining. B, lysates of nocodazole-arrested mitotic HeLa cells were subjected to pulldown by GST or the indicated GST fusion proteins followed by anti-INCENP immunoblotting and Coomassie Brilliant Blue staining. C and D, lysates of nocodazole-arrested mitotic HeLa cells were subjected to pulldown by MBP or the indicated MBP fusion proteins followed by anti-HP1α (C and D), -HP1β, or -HP1γ (D) immunoblotting and Coomassie Brilliant Blue staining. E, lysates of nocodazole-arrested mitotic HeLa cells stably expressing INCENP-GFP were subjected to anti-GFP immunoprecipitation (IP) followed by immunoblotting with the indicated antibodies. F, lysates of nocodazole-arrested mitotic HeLa cells transiently expressing HP1α-FLAG and INCENP-GFP (WT or the indicated mutants) were subjected to anti-FLAG immunoprecipitation followed by anti-FLAG or anti-GFP immunoblotting.