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All cysteine variants bind Zn2+. A–C, release of Zn2+ from denatured OstR-C3A, OstR-C4A, and OstR-C169A, respectively, determined by absorbance of Zn2+ in complex with PAR (peak absorbance at 520 nm). D, identified cysteine mutants were incubated with 2 mm ZnCl2 for 30 min followed by treatment with 12 mm H2O2 for another 30 min (lanes 3, 5, and 7). Lanes 2, 4, and 6, Zn2+-treated OstR-C3A, OstR-C4A, and OstR-C169A, respectively.
