Oxidation of OstR cysteine variants by H2O2 and CuCl2.
A, C, and E, OstR-C3A. OstR-C4A and OstR-C169A oxidized with increasing concentration of H2O2 (10 μm to 2 mm). B, D, and F, OstR-C3A. OstR-C4A and OstR-C169A oxidized with increasing concentration of CuCl2 (10 μm to 2 mm). Lanes 1, protein marker (kDa); lanes 2, reduced protein (Rd); lanes 3, air-oxidized protein (O2), except for B, in which air-oxidized protein is not shown.