Table 1.

Summary of cyt c modifications identified by gel-based bottom-up LC-MS/MS experiments in the absence, or presence of H₂O₂, TLCL or combination of both

Cyt c (100 μm) was incubated in ammonium bicarbonate buffer alone, in the presence of H₂O₂ (1mm), TLCL (10 mm) or combination of both for 30- and 60-min. Proteins were separate by SDS-PAGE, digested with trypsin and analyzed by LC-MS/MS. PTMs were identified from obtained tandem mass spectra using search engine–assisted database search.