Figure 2.

Apoptotic/Necroptotic Effects in H9c2 Cells under Hypoxic Stimulation

(A) Cell viability against hypoxic stimulation (n = 3). (B) Flow cytometry analysis of apoptosis/necroptosis of H9c2 cells under hypoxic stimulation using Annexin V-PI (n = 3). (C) Differentiation of apoptosis/necroptosis markers and death receptors in response to hypoxic stimulation (n = 4). (D) Band intensities of apoptosis and necroptosis markers. The values given were normalized to the band intensity of β-actin as an internal control. *p < 0.05; **p < 0.01; n = 3. (E) Alteration of RIP3 and BNIP3 expression levels in response to hypoxic stimulation in H9c2 cells (n = 3). (F) Representative immunocytochemistry images of apoptosis (BNIP3 and CASP3) and necroptosis (RIP3 and p-MLKL) markers. Scale bars, 20 μm. The data are normalized to the 0-h control. The data are presented as the mean value ± SD of three separate experiments. n = 3.