Figure 5.

Anti-necroptotic/Anti-apoptotic Functions of miR-105 in MI Rat Hearts

(A) Representative western blot bands showing apoptosis and necroptosis markers. GAPDH was used as an internal control to normalize the expression of the target genes. n = 4. (B) Representative immunofluorescence images of staining with TUNEL (apoptotic cells), PI (necroptotic cells), and DAPI. Scale bars, 200 μm. n = 3. (C) Histological analysis of MI rat hearts after miR-105 injection. Cardiac fibrosis was evaluated by Masson’s trichrome staining. n = 3. (D) Cardiac function analysis. EF, ejection fraction; ESV, end-systolic volume; V@dP/dt min, volume at dP/dt min. n = 3 independent experiments.