Skip to main content
. 2019 Feb 4;7:7. doi: 10.3389/fcell.2019.00007

FIGURE 2.

FIGURE 2

CDC42 effectors partially rescue post-Golgi transport of VSVG in FGD1-deficient HeLa cells. HeLa cells were treated with targeted FGD1 siRNA and co-transfected with VSVG-GFP and a plasmid encoding CDC42 effector (indicated at the corresponding panel). Cells were subsequently exposed to the 20°C block for 2 h and then shifted to 32°C for 60 min in the presence of tannic acid. (A) Expression of either the PAK1 or the IQGAP1 mutant partially rescued the VSVG-GFP transport in FGDY cells; (B) Quantification of post-Golgi VSVG-GFP- containing carriers per cell in the experiments shown in panel A (n = 30 cells; ∗p < 0.005, t-test). (C) Co-expression of VSVG-GFP and different constitutively active CDC42 mutants (see Table 1) partially rescued transport of VSVG-GFP in FGD1-silenced cells. (D) Quantification of post-Golgi VSVG-GFP-containing carriers per cell in the experiments shown in panel C (n = 30 cells; ∗∗∗p < 0.001, ∗p < 0.005, t-test). Scale bars, 20 μm.