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. 2019 Feb 4;7:7. doi: 10.3389/fcell.2019.00007

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Copyright © 2019 Egorov and Polishchuk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of CLASP1 distribution in FGD1-deficient cells. HeLa cells were transfected with FGD1-specific siRNAs and stained with antibodies against CLASP1 and TGN46. Control cells (A,B) exhibited a faint CLASP1 signal at the Golgi, while CLASP1 levels significantly increased in FGD1-deficient cells (D,E). Quantification (C,F) showing CLASP1 and TGN46 fluorescence in the areas outlined by dashed boxes in panels B (control cells) and D (FGD1-deficient cells). Scale bar, 28 μm.