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. 2018 Dec 13;27(2):314–325. doi: 10.1016/j.ymthe.2018.11.014

Figure 1.

Figure 1

Design and Expression of Murine FSHR Native and SynCon Vaccines

(A) Depiction of murine FSHR native and consensus vaccines and stretches encompassed by the peptide pools. Mutations for FSHR-signaling ablation are depicted in red, and mutations for the SynCon sequence are depicted in black. (B) Schematic subcloning strategy into modified pVAX. (C) Immunofluorescence of 293T cells transfected with murine FSHR consensus vaccine, murine native FSHR vaccine, or modified pVAX empty vector stained with polyclonal anti-mouse FSHR antibody (representative of 2 independent experiments). *Mutation. Scale bars, 10 μm.