Figure 4.

Murine FSHR SynCon Vaccine Generates a Long-Lasting Cellular Response

(A) Schematic of immunization protocol. IFNγ ELISPOT of splenocytes from mice immunized with murine FSHR SynCon vaccine, native FSHR vaccine, or empty vector pulsed with FSHR-derived peptides is shown as (B) total spots or (C) aggregated per peptide pool. (D–I) Percentages of (D and G) IFNγ, (E and H) TNF-α, and (F and I) IL-2 produced by CD8⁺ and CD4⁺ T cells from the spleen of mice immunized with mouse FSHR SynCon vaccine, native FSHR vaccine, or empty vector, stimulated with murine FSHR-derived peptides. (Pool of 2 independent experiments with a total of n = 7–9 mice per group.) ANOVA. Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001.