Figure 2.

NF-κB and NFAT Activation in Jurkat Cells Detected with Reporter Genes

(A) Schematic representation of reporter cells with NF-κB- and NFAT-inducible ECFP and EGFP reporter genes, respectively. RE, response element; MP, minimal promoter. (B) MFI of ECFP and EGFP after stimulation of reporter cells with PMA and ionomycin (P/I). (C) Phenotype of reporter cells and BW5147 stimulator cells. The membrane-bound anti-CD3-scFv of BW-OKT3 cells was detected by its CD14 stem domain. Numeric values represent the MFI of transduced cells minus the MFI of control. (D) Co-culture of reporter cells with BW5147 stimulator cells distinguished by human CD45 detection during flow cytometry analysis. (E) MFI of ECFP and EGFP after stimulation of reporter cells with BW-OKT3 cells at a 2.5:1 ratio for 24 hr.