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The smooth muscle layer-like phenotype generated by the dental pulp stem cell (DPSC) clone A32 after SMC induction. After a long period of differentiation (20 days), the induced A32 formed a monolayer and generated the smooth muscle-like phenotype which was positive for expression of the SMC markers, α-SMA (b–d), myosin (f–h), and desmin (j–l). Noninduced A32 as the negative control (a, e, i). The green staining indicates a positive result. Nuclei were stained with DAPI. Bars: 50 μm.
