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. 2019 Jan 28;2019:8907570. doi: 10.1155/2019/8907570

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Copyright © 2019 Wenkai Jiang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The involvement of Wnt-mediated GSK3β/β-catenin in SMC differentiation from DPSC clone A32. The A32 clone was induced according to the SMC induction protocol, 20% conditioned medium, and 2.5 ng/mL TGF-β1 for the indicated time (0 d, 5 d, 8 d, 11 d, and 14 d). The protein levels of p-GSK3β, t-GSK3β, and β-catenin were analysed with western blotting (a and c). The relative band intensities were determined by densitometry (b and d). Statistical analysis was performed by using one-way ANOVA. Date are shown as means ± SEM. ^∗P < 0.05 when compared with the 0 d group.