Table 1.

List of divergent primers designed for use in confirmation of putative circRNA.

Divergent primer	Sequence
HindIII 10	Forward: 5′-CTATAGTTGAAGCACCTGATGGTGT-3′
	Reverse: 5′-GAGCCATAAAGATAGGCAGTAACTACA-3′
HindIII 33	Forward: 5′-TGGTTCACCACAACCCGT-3′
	Reverse: 5′-TGTGTGACTCAAGTTCTCAGTTTGTAA-3′
osi_circ1 (1:36416264-36418547)	Forward: 5′-TGGTAGCAACCGCACAAA-3′
	Reverse: 5′-ATGCTTCCAGGCACATCA-3′
osi_circ2 (2:19273316-20009087)	Forward: 5′-GGGAGCTCAAGGTGAAGAT-3′
	Reverse: 5′-GTTGAACAAACAACACACAAC-3′
osi_circ3 (8:24552647-24573025)	Forward: 5′-ACGTTGAGAGTAAGTTTCCG-3′
	Reverse: 5′-CCCTTTACGATACCACTAGCC-3′
osi_circ4 (12:16650523-17328210)	Forward: 5′-TAGGCTCACGATGTGTTGC-3′
	Reverse: 5′-CGATGAGGGCTGCGAAC-3′
osi_circ5 (9:15720676-15721227)	Forward: 5′-ATCCTTGGAGCTGGCTATGA-3′
	Reverse: 5′-ATCTCGGTTGACCACACACT-3′
osi_circ6 (7:15534138-15534682)	Forward: 5′-TCAAGTCCGCCGTCAAATC-3′
	Reverse: 5′-CCCAAGGGCAGGTTCTTAC-3′
osi_circ7 (6:27117575-27118530)	Forward: 5′-TGCAGAAACAGCATGGTCA-3′
	Reverse: 5′-ATAGGGTGCAAACCTGTGAG-3′
osi_circ8 (8:4494958-4495647)	Forward: 5′-AGAGTCTCTGGCAGTCTCC-3′
	Reverse: 5′-AACCAGTGACTAGCAACTAAGAA-3′
osi_circ9 (1:41518651-41519075)	Forward: 5′-GCGACCTTACTGCACGAATA-3′
	Reverse: 5′-TTGCAAGCGCAACACAAC-3′
osi_circ10 (8:15854661-15861841)	Forward: 5′-GCTAGCAGGGACAGGTTATC-3′
	Reverse: 5′-CAGAAGACGTGTGTGCCTAT-3′
Nb_circ1 (Niben101Scf01334:583095–583645)	Forward: 5′-CTGGGTCAGTCCTCCATTT-3′
	Reverse: 5′-AGATACGCATGCCTCCAA-3′
Nb_circ2 (Niben101Scf01481:214685-215144)	Forward: 5′-TCAACGTGCTTCCTGAACT-3′
	Reverse: 5′-AAATGCTTGGGTCCTACTCC-3′
Nb_circ3 (Niben101Scf01671:738307-738555)	Forward: 5′-TCTTGTCCCAGTCCAGAGA-3′
	Reverse: 5′-TGTCTCCGCGTGTTAATGT-3′
Nb_circ4 (Niben101Scf01820:33613-33924)	Forward: 5′-GTTGTGCTCATTCCATTGGG-3′
	Reverse: 5′-TGCTTCCTGAGCAAGTTCTG-3′
Nb_circ5 (Niben101Scf01505:317983-318653)	Forward: 5′-CCCAATCCACCTTGATCCTT-3′
	Reverse: 5′-CACGACTGGATTTGGCGATA-3′
Nb_circ6 (Niben101Scf32276:10732-11201)	Forward: 5′-TGGGTACCGAAGTGTACTGT-3′
	Reverse: 5′-AAACCTTGGACCGAGATCAAAT-3′
Nb_circ7 (Niben101Scf27324:1438–11811)	Forward: 5′-TGAGCCATTCGCAGTTTCA-3′
	Reverse: 5′-GGTCGTCTCGTCCCTTCT-3′
Nb_circ8 (Niben101Scf15187:11992–12579)	Forward: 5′-TGGCTAGAATGCGGGTTTC-3′
	Reverse: 5′-ATCTTGAAAGTCGTGGTTTCCT-3′
Nb_circ9 (Niben101Scf09703:266605–266722)	Forward: 5′-GCAGTTGGAGACTTTGAGGT-3′
	Reverse: 5′-TGCCGCAAGGGTGATATG-3′
Nb_circ10 (Niben101Scf11535:99376–100075)	Forward: 5′-ACAGGTAGTCTGTTCCGACA-3′
	Reverse: 5′-AGATGCCGAGGAGTTGGA-3′