Figure S8. DM-α-KG treatment does not significantly affect methylation marks.

(A) Methylated histone marks were probed in rho0 cells exposed or not to DM-α-KG; N = 4 independent experiments labeled as R1–R4. (B) Same as (A) but using DN-POLG cells at days 0 and 9, N = 3 depicted as R1–R3. (C) Levels of 5hmeC were estimated in rho0 prior and after exposure to DM-α-KG using dot blots. Samples were spotted in quadruplicates; N = 4. Graph depicts the average of the four each independent replicate ± SEM. No primary antibody was used as control (omitted).