Fig. 3.

A selective inhibitor of Axl kinase decreased PD-L1, PD-L2 and CXCR6 mRNA expression in vitro. Relative expression values of CD274 (PD-L1; a, d), PDCD1LG2 (PD-L2; b, e) and CXCR6 (c, f) in PC9 cells (a-c) and H1975 cells (d-f) six hours after treatment with 0.1% DMSO or Axl inhibitor BGB324 (10 μM). Each value represents the fold-increase in gene expression of BGB324-treated cells compared to that of DMSO-treated cells in each experiment. Data are expressed as the mean ± SD of three independent experiments. ****p < 0.0001, **p < 0.01, *p < 0.05, vs. DMSO; two-tailed paired t-test. NS indicates not significant