Figure 4.

Putative Kir7.1 Loss-of-Function Rescue Through Nonsense Suppression

(A–C) (A) Average I/V relationship before (red) and after (dark blue) treatment with NB84. The Rb⁺ current measured is shown as a light-blue trace. Evaluation of the average inward current measured at −150 mV (B) and of the membrane potential (C) to demonstrate the effect of NB84.

(D) Immunoblot showing detection of eGFP fusion proteins, via anti-GFP antibody, in cell lysates from CHO cells transfected with empty vector (GFP), wild-type Kir7.1 coding sequence (WT), or Trp53^∗ coding sequence (Mut). A partial restoration of the full-length protein product was observed in the latter after NB84 treatment (Mut + NB84).