Figure 5.

Scaffold-Mediated Non-viral Delivery of miR-219/miR-338 Supported Myelin Regeneration and Axon Ensheathment within Scaffold

(A) Representative images comparing different miR-219/miR-338- and Neg miR-loaded scaffolds. Relatively prominent MBP and CNPase signals were observed within scaffolds with miR-219/miR-338 at week 4. White dotted lines demarcate the region occupied by the scaffold. Scale bar represents 250 μm. (B) Percent of fiber-scaffold area that is MBP⁺. (C) Representative images from week 4 samples showing MBP⁺ signals in scaffold. White arrows indicate axons that appeared to be partially ensheathed. Scale bar represents 20 μm in the normal images and 10 μm in the magnified images. (D) Myelination index obtained within the scaffolds. **p < 0.01, *p < 0.05 (Student’s t test).