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. 2018 Dec 18;9(2):890–894. doi: 10.1021/acscatal.8b03752

Figure 2.

Figure 2

Photoenzymatic hydroxylation of ethylbenzene to (R)-1-phenylethanol using 5 μM FMN (orange ⧫), 10 μM methylene blue (red ▲), 5 μM phenosafranine (green ■), or 10 μM methylene blue + 5 μM phenosafranine + 5 μM FMN (□) as photocatalysts. Conditions: c(rAaeUPO) = 100 nM, c(CbFDH) = 4.8 μM, c(NAD+) = 0.4 mM, c(NaHCO2) = 75 mM, c(ethylbenzene) = 10 mM, 50 mM KPi buffer (pH 7, 0.8% MeOH (v/v)), T = 30 °C, λ = 450, 520, and 630 nm (blue, green, and red LED light). Please note: using a broadspectrum (sunlight-imitating) light source (Lightincure LC8 L9566, Hamamatsu) gave comparable results (Figure S9).