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. 2018 Dec 18;9(2):890–894. doi: 10.1021/acscatal.8b03752

Figure 3.

Figure 3

Influence of different reaction parameters on the product formation rate of the photoenzymatic hydroxylation of ethylbenzene. (A) Concentration of the photocatalyst (red ■: methylene blue; green ⧫: phenosafranine; orange ▲: FMN); (B) intensity of broadband light source (c(rAaeUPO) = 100 nM, c(CbFDH) = 4.8 μM, c(NAD+) = 0.4 mM); concentration of rAaeUPO (C), CbFDH (D), or NAD+ (E). Conditions (unless indicated otherwise): c(rAaeUPO) = 50 nM, c(CbFDH) = 2.4 μM, c(NAD+) = 0.2 mM, c(NaHCO2) = 75 mM, c(ethylbenzene) = 10 mM, 50 mM KPi buffer (pH 7, 0.8% MeOH (v/v)), T = 30 °C.