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. 2018 Nov 13;27(2):355–364. doi: 10.1016/j.ymthe.2018.11.005

Figure 4.

Figure 4

SETDB1 Serves as a Direct Target Gene of miR-621

(A) SETDB1 mRNA expression in 50 paired HCC tumor and adjacent nontumor tissues based on TCGA dataset. (B) SETDB1 protein expression (detected by IHC) in 50 paired HCC tumor and adjacent nontumor tissues based on our dataset. (C) SETDB1 expression in the human HCC lines HepG2, Smmc-7721, and Bel-7404 and the normal liver cell line LO2 was detected by real-time qPCR. (D) The construction of psiCheck2 report plasmid, containing WT or Mut SETDB1 3′ UTR. (E) The luciferase assay of HepG2 cells transfected with WT or Mut SETDB1 3′ UTR luciferase vectors and miR-621 mimic or miR-NC. The luciferase activity was measured at 24 hr after transfection. *p < 0.05, **p < 0.01, ***p < 0.001.