Figure 6.

SETDB1 Reverses the Effects of miR-621 on Cell Radiosensitivity

HepG2 (A) and Smmc-7721 (B) cells transfected with miR-NC + vector control, miR-621 mimic + vector control, miR-NC + SETDB1 without 3′ UTR, or miR-621 mimic + SETDB1 without 3′ UTR were irradiated with a range of 2 Gy to 8 Gy IR doses. After 14 days, colonies were stained and scored for colony formation, and the SF was plotted as described in the Materials and Methods. (C) The expression of γ-H2AX protein was detected 6 h after IR by western blotting. (D) Quantification of γ-H2AX protein. (E) Quantification of tail moment. (F and G) Treated cells were cultured for 24 hr, harvested, stained with Annexin V-FITC/PI, and then analyzed using flow cytometry. Representative images (F) and quantitative data (G) are shown. Each column is shown as the means of three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.