Figure 4.

The Cortex and Striatum Are Unaffected by GRN Overexpression

(A) The ipsilateral cortex immediately adjacent to the degenerated hippocampus appeared unaffected by AAV9.GRN overexpression when compared to the contralateral cortex and to cortex ipsilateral to AAV9.eGFP-injected brain. Gross morphology, layer organization, and neuronal numbers appeared unremarkable by H&E staining (B; n = 10 AAV9.GRN-injected mice, and n = 5 AAV9.eGFP-injected mice assessed); NeuN staining confirmed intact neuronal appearance (C). (Scale bars: 100 μm.) Similarly, the ipsilateral striatum directly anterior to the degenerated hippocampus (D) appeared unremarkable by H&E (E), NeuN (F), and GFAP (G) staining, compared to AAV9.eGFP-injected controls 6 months post-injection (scale bars: 100 μm), as well as to uninjected controls (data not shown).