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. 2018 Sep 6;27(2):380–393. doi: 10.1016/j.ymthe.2018.08.026

Figure 3.

Figure 3

Regulatory Mechanism of lncITPF in Its Host Gene

(A) Itgbl1 expression was higher in fibrotic tissue than in normal tissue on the basis of RNA sequencing. (B) Itgbl1 was upregulated in cells treated with 5 ng/mL TGF-β1 for 12, 24, 48, and 72 hr. (C) qRT-PCR analysis showed that Itgbl1 was decreased by lncITPF RNAi and increased by lncITPF overexpression. (D) Western blot showed that ITGBL1 was decreased by lncITPF RNAi and increased by lncITPF overexpression. (E) Single-molecule RNA-FISH detecting the location of lncITPF (red) in cells. U6 and 18S RNA were used as cytoplasmic and nuclear localization markers, respectively. DNA (blue) was stained with DAPI. A representative image is shown. (F) qRT-PCR analysis of RNAs purified from nucleoplasmic (red), chromatin nuclear (gray), and cytosolic (blue) compartments in cells. NC indicates a negative control, BP indicates blank plasmid, and RP indicates the recombinant plasmid of the overexpressed lncITPF. Each bar represents the mean ± SD; n = 6; **p < 0.01 and ***p < 0.001 versus the control group.