Fig. 4. Binding response can be observed at low concentrations of spy molecule 19 and protein. (a) Overlay of the 19F CPMG peak (200 ms filter) of spy molecule 19 in the absence (blue) and presence of protein (red). When lowering the concentrations of spy molecule and protein, the S/N and C2 values respectively decreased. (b) Measurement of the C2 of spy molecule 19 at different concentrations (in triplicates), in the presence of four concentrations of protein. The error progressively increases as the concentration of spy molecule goes down, due to the increase in noise of the NMR data.
