Fig. 5.

rBiFC analysis of self-interaction and nuclear foci localization of phosphomimic and phospho-null AHL10. (A) Quantification of relative self-interaction intensity for phosphomimic and phospho-null AHL10. Data are means ± SE (n = 10–20) combined from two independent experiments. None of the phosphomimic or phospho-null constructs differed significantly from wild type (N.M) AHL10 in either stress or control treatments (t-test P ≤ 0.05). Images of AHL10 self-interaction rBiFC assays are shown in SI Appendix, Fig. S13. (B) Representative images of nuclear foci localization of AHL10 self-interaction complexes observed in rBiFC assays. For N.M. wild-type AHL10 and phosphomimic AHL10^S313D and AHL10^S314D, representative images of nuclei without foci are shown in SI Appendix, Fig. S13B. Note that nuclei with foci were never observed for AHL10^S314A. (Scale bars, 5 μm.) (C) Portion of nuclei with AHL10 foci. Individual nuclei (80–130 for each construct and treatment, combined from two independent experiments) were counted. Error bars indicate 95% confidence intervals, and asterisks indicate significant difference compared with wild type in the same treatment (or difference of wild-type stress versus control) based on Fisher’s exact test (P ≤ 0.05).