Fig. 2.

Jugular vein hemostatic plug morphology: extravascular side. (A) Low-magnification SEM image of a representative hemostatic plug fixed 5 min postinjury. (Scale bar: 300 µm.) The sample is tilted to provide a profile view. (B) The same hemostatic plug shown in A viewed from above. (Scale bar: 300 µm.) Location of the zoomed image shown in D is indicated. (C) Corresponding fluorescence image of the same hemostatic plug shown in A and B, oriented as in B. The image is a maximum-intensity projection of a series of z-plane images acquired using 2-photon microscopy. A 3D reconstruction of the fluorescence images is included in Movie S2. Dotted line in B and C indicates the injury site. (Scale bar: 300 μm.) (D) Higher-magnification image of the region indicated in B, showing abundant highly activated platelets interspersed with fibrin at the injury site. (Scale bar: 50 µm.) (E) Higher-magnification image of the region indicated in D showing highly activated platelets. (Scale bar: 10 µm.) Some fibrin is also seen at the lower right of the image. Asterisk notes an intact platelet in the same field of view for comparison. Micrographs shown are representative of >10 hemostatic plugs imaged from the extravascular side 5 min postinjury. An interactive image file presenting multiple perspectives of the hemostatic plug shown is included in the SI Appendix (Dataset S2).