Fig. 2.

Symptom expression and RNA accumulation of CHV1-Δp69 in the C. parasitica mutants with deletions in the genes up-regulated by viral infection. (A) Phenotypic growth of DK80 and its disruption mutants of virus-induced genes (Δuprt, Δgtpch, Δaprt, Δhp1, Δhp2, Δbtb, Δhel, and Δac) infected with CHV1-Δp69. The fungal colonies were cultured on PDA plates for 5 d and photographed. See SI Appendix, Fig. S5 for colony morphologies of a subset of the virus-free mutant strains. (B) Colony size of the CHV1-Δp69–infected fungal mutants shown in A. Bars represent colony diameter (n = 3). Asterisk (*) indicates a significant difference (Student’s t test) at P < 0.1. (C) Northern blotting analysis of the CHV1-Δp69 RNA genome and dcl2 transcripts in the fungal mutants shown in A.