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. 2019 Jan 23;116(6):2274–2281. doi: 10.1073/pnas.1812407116

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Fig. 4. — Complementation using DCL2 homologs from other fungi. (A) Schematic diagrams showing helicase (DEXDc and HELICc), ribonuclease III (RIBOc), and DSRMs in DCL2 proteins from C. parasitica (ABB00357), R. necatrix (GAP83771), and F. graminearum (XP_011321198). (B) Phenotypic growth of C. parasitica Δdcl2 expressing homologous DCL2 from C. parasitica (+Pcrp:Cp_dcl2), and heterologous DCL2 from R. necatrix (+Pcrp:Rn_dcl2) or from F. graminearum (+Pcrp:Fg_dcl2) infected with CHV1-Δp69. The fungal strains were cultured on PDA plates for 7 d and photographed. (C) Northern blotting analysis of the CHV1-Δp69 RNA genome and uprt gene transcript accumulation in the fungal strains described in B.