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. 2019 Jan 18;116(6):2226–2231. doi: 10.1073/pnas.1818865116

Fig. 2.

Fig. 2.

Inhibitory effects of GHRH antagonists in MPM cells. Cell survival (A and C) and proliferation (B and D) assessed by MTT and BrdU assays, respectively, in MSTO-211H and REN cells untreated or treated for 72 h in medium with 2.5% serum (c, control medium) with 1 μM MIA-602, alone or with PEM, at the concentrations indicated. Results, expressed as percent of control, are mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. c; #P < 0.05, ##P < 0.01; n = 4. Caspase-3 activity in MSTO-211H (E) and REN cells (G) treated for 24 h with 1 µM MIA-602 or MIA-690. Results, expressed as percent of control are mean ± SEM. *P < 0.05 and **P < 0.01 vs. control; n = 3. Representative Western blot for Bcl-2 (F and H), p53 (I and K), and c-Myc (J and L) in cells treated with 1 µM MIA-602 or MIA-690 for the indicated times. Results, normalized to actin and expressed as percent of control (Time 0), are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001; n = 3. ns, not significant.