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. 2019 Jan 23;116(6):2193–2199. doi: 10.1073/pnas.1819020116

Fig. 2.

Fig. 2.

prp-8 alleles map to conserved regions of Prp8. (A) ClustalOmega (33) alignment of Prp8 sequences showing conservation (blue) in the region of az24 (yellow) and az43 (orange). (B) S. cerevisiae C complex structure (5LJ3) (7) highlighting Prp8’s (tan) central position, Snu114 (green), U5 snRNA (blue), and U6 snRNA (red). These interactions are virtually identical in all post B-complex structures. (C) Zoom to spliceosome’s catalytic core. Catalytic Mg2+, magenta. Positions of Prp8-T607 (orange) and Prp8-G736 (yellow) are shown. (D) Prp8-T607 (orange) contacts the intron (black) 5′ SS +3 phosphate. (E) Prp8-G736 (yellow) is part of a structured loop (edges indicated by asterisks) that forms during activation. This loop acts as a brace, supporting U6 snRNA.