Table 2.
Cryptic splicing measurements of the unc-73(e936) gene in the presence of the PRP-8 G654 substitution alleles
| Strain | Genotype | PRP-8 | “+23”, % | wt, % | “−1”, % | Thrashes/min |
| SZ181 | unc-73(e936); prp-8(+) | wt | 72.9, 73.7 | 12.1, 13.8 | 14.9, 12.5 | 10.2 ± 2.9 |
| SZ155 | unc-73(e936); prp-8(az29) | G654E | 54.3, 53.7 | 27.1, 24.9 | 18.6, 21.4 | 108.4 ± 9.9 |
| SZ255 | unc-73(e936); prp-8(az75) | G654K | 64.5, 62.6 | 19.5, 19.7 | 16.0, 17.7 | 50.8 ± 8.0 |
| SZ256 | unc-73(e936); prp-8(az76) | G654L | 56.7, 56.5 | 22.5, 23.7 | 20.8, 19.7 | 59.8 ± 12.7 |
| SZ257 | unc-73(e936); prp-8(az77) | G654P | 63.4, 62.0 | 20.1, 21.8 | 16.6, 16.2 | 41.4 ± 8.8 |
| SZ258 | unc-73(e936); prp-8(az79) | G654I | 59.3, 59.4 | 21.7, 21.8 | 19.0, 18.8 | 40.6 ± 10.1 |
| SZ259 | unc-73(e936); prp-8(az80) | G654C | 65.8, 62.6 | 20.4, 20.6 | 13.8, 16.7 | 33.4 ± 5.9 |
| SZ260 | unc-73(e936); prp-8(az82) | G654V | 57.4, 57.4 | 23.3, 23.3 | 19.3, 19.3 | 43.8 ± 5.5 |
| SZ261 | unc-73(e936); prp-8(az83) | 8aa ins. | 62.9,61.7 | 21.7, 23.4 | 15.4, 14.9 | 85.8 ± 15.9 |
| N2 | unc-73(+); prp-8(+) | wt | 100.0 | 133.4 ± 11.1 |
The last column is a thrash assay in which L4 worms were placed in a drop of M9 medium on a glass slide and the number of times that they bent their body across the central axis in 1 min was measured. Mean and SD of 10 independent trials listed.