Table 5.
Analysis of alternative 3′ splicing events predicted from mRNA-Seq analysis of the prp-8 T524S mutant libraries
| Genotype | Up 3′ SS use strain 1,2, % | |
| Gene & Sequence: cdt-2 aaatcaaaaag▲ATTTCAG△TTTTTCCCGC | ||
| prp-8(+) | 18.24, 18.41 | |
| prp-8(G654E) | 27.65, 28.28 | |
| prp-8(T524S) | 51.51, 52.45 | |
| Gene & Sequence: T10E9.14 atattttgcag△TTTCAG▲GAAACTATCAAC | ||
| prp-8(+) | 16.76, 17.72 | |
| prp-8(G654E) | 19.34, 20.04 | |
| prp-8(T524S) | 5.44, 4.58 | |
| Gene & Sequence: F48D4.6 gttattcag▲TGTTCCAG△AAACAG△TGACGACTT | ||
| prp-8(+) | 54.90, 53.69 | |
| prp-8(G654E) | 49.98, 51.13 | |
| prp-8(T524S) | 79.53, 79.69 | |
| Gene & Sequence: W09G3.7 atcacgaatttag△CTTCCAG▲ATCGGTGGT | ||
| prp-8(+) | 84.59, 83.27 | |
| prp-8(G654E) | 81.61, 81.82 | |
| prp-8(T524S) | 62.01, 60.82 | |
| Gene & Sequence: dct-1 aacgcttcag△GTATGTCAG▲AATCGTGGGTGGAA | ||
| prp-8(+) | 62.48, 56.68 | |
| prp-8(G654E) | 48.90, 50.14 | |
| prp-8(T524S) | 44.33, 45.60 | |
Five of the 10 alternative 3′ splicing events were tested. Sequences of the alternatively spliced regions are shown, with the 3′ SSs promoted in the prp-8 mutant indicated by a filled triangle, and the partner 3′ SS by an open triangle (in F48D6.4 there are three alternative 3′ SSs). Uppercase indicates sequences that can be exons, while lowercase are always intronic. Below the sequence is quantitation of relative alternative 3′ SS usage by 32P-RT-PCR analysis (SI Appendix, Fig. S5). Genotypes are indicated.