Table 2. Overview of the two staining panels each dedicated to a specific cell type which is indicated by individual colors.
| Excitation (nm) | Blue: 488 | Red: 633 | Violent: 405 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Emission (nm) | 523 | 575 | 613 | 692 | 760 | 650 | 720 | 767 | 455 | 528 |
| Fluorochrome | FITC | PE | ECD | PE-Cy5.5 | PE-Cy7 | APC | AF700 | AA750 | PB | KRO |
| Panel 1 | CD45RA | CCR7 | CD28 | PD1 | CD27 | CD4 | CD8 | CD3 | CD57 | CD45 |
| CC of panel 1 | CD4 | CD4 | CD28 | PD1 | CD27 | CD4 | CD8 | CD3 | CD4 | CD8 |
| Panel 2 | TCRγδ | TCRαβ | HLA-DR | – | TCR Vδ1 | CD4 | CD8 | CD3 | TCR Vδ2 | CD45 |
| CC of panel 2 | CD4 | CD4 | HLA-DR | – | TCR Vδ1 | CD4 | CD8 | CD3 | CD4 | CD8 |
Note:
FITC, Fluorescein isothiocyanate, PE, Phycoerythrin; ECD, Phycoerythrin-Texas Red-X; PE-Cy, Phycoerythrin-Cyanine; APC, Allophycocyanin; AF700, Alexa Flour 700; AA750, Allophycocyanin Alexa Flour 750; PB, Pacific Blue; KRO, Krome Orange; CC, compensation control.