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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: J Immunol. 2019 Jan 16;202(4):1229–1238. doi: 10.4049/jimmunol.1801227

Figure. 1: AG-30/5C induces MC degranulation via MRGPRX2:

Figure. 1:

(A) LAD2 cells were stimulated with different concentrations of AG-30/5C (0.01 – 1.5 μM) for 30 min. Percent degranulation (β-hexosaminidase release) was determined. (B) LAD2 cells were stably transduced with scrambled control shRNA or lentivirus shRNA targeting human MRGPRX2. Western blotting was performed to determine MRGPRX2 expression. β-actin was used as a loading control. (C) Control and MRGPRX2 knockdown LAD2 cells were stimulated with AG-30/5C (1 μM) and degranulation was determine. (D) RBL-2H3 and RBL-2H3 cell stably expressing MRGPRX2 (RBL-MRGPRX2) were stimulated with different concentrations of AG-30/5C (0.01 – 1.5 μM) and percent degranulation was determined. All data are expressed as a mean ± SEM of three experiments. Statistical significance was determined by non-parametric t-Test and two way ANOVA (P value<.05). Western blotting data presented are representative of three similar experiments. *** indicates P value<.001,** indicates P value <.01 and * indicates P value <.05.