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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: J Immunol. 2019 Jan 16;202(4):1229–1238. doi: 10.4049/jimmunol.1801227

Figure. 2: Pertussis toxin inhibits AG-30/5C and compound 48/80-induced MC degranulation but not calcium mobilization.

Figure. 2:

(A) RBL-MRGPRX2 cells cultured in absence (-PTx) or presence of pertussis toxin (+PTx, 100 ng/ml, 16h). Cells were stimulated with compound 48/80 (1μg/mL) or AG-30/5C (1 μM) and percent degranulation was determined. (B-C) RBL-MRGPRX2 cells were cultured in the absence or presence of PTx, loaded with Indo-1 (1 μM, 30 min) and stimulated with either compound 48/80 (1 μg/mL) or AG-30/5C (1 μM) as indicated by arrows and time course of calcium mobilization was determined. Representative traces of 3 similar experiments are shown. All data points for degranulation are expressed as mean ± SEM of three experiments. Statistical significance was determined by non-parametric t-Test and two way ANOVA (P value<.05). *** indicates P value<.001,** indicates P value <.01 and * indicates P value <.05.