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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: J Immunol. 2019 Jan 16;202(4):1229–1238. doi: 10.4049/jimmunol.1801227

Figure. 4: Nateglinide is a balanced agonist whereas AG-30/5C is a G protein -biased agonist for MRGPRX4.

Figure. 4:

(A) Untransfected (HTLA) and stably transfected HTLA-MRGPRX4-Tango (HTLA-MRGPRX4) cells were incubated with anti-FLAG-PE antibody and receptor expression was determined by flow cytometry. A representative overlay histogram of MRGPRX4 expression is shown. (B) Effects of nateglinide (30 μM), AG-30/5C (10 μM), compound 48/80 (30 μg/mL) on β-arrestin-mediated gene expression was determined as described in Figure 3. (C) Representative Ca2+ measurements traces in response to high concentrations of nateglinide (30 μM), AG-30/5C (10 μM) and compound 48/80 (30 μg/mL) are shown. All data points for the Tango assay are expressed as a mean ± SEM of three experiments. Statistical significance was determined by non-parametric t-Test and two way ANOVA (P value<.05). *** indicates P value<.001,** indicates P value <.01 and * indicates P value <.05.