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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Insect Biochem Mol Biol. 2018 Sep 29;104:91–105. doi: 10.1016/j.ibmb.2018.09.011

TABLE 1.

Cuticular proteins identified in different structures

Updated data from references below.
Source of data This paper He et al. 2007 Zhou et al. 2016 2017
Protein name seq cluster Prob Palp Larva - terminal seg Larva - whole 4th Larval tracheae Larval head capsules Cast pupal cuticles Antenna with JO* Eye lens Leg Wing whole adults Up-to-date summary
CPR1 2RA US US US S US US US US
CPR2/4 S S U US S S S S US
CPR3 US US US S US US S S US
CPR5 S S S S S S S S S
CPR6 US US US US S S S S US
CPR7 no
CPR8 [fUmU1] U U U
CPR9-PA-PB bU mU U U U U U U U U
CPR10 U U U U U
CPR11 U U U1 U U
CPR12/13 U U U US S US U US
CPR14 U1 U U
CPR15 bU mU1 U U US S US US U U U US
CPR16 bU bU U U U1 U U U U U U U
CPR17 2LA no
CPR18 no
CPR19 no
CPR20 no
CPR21 fU1 U U U1 U U U
CPR22 U U U1 V&W U
CPR23 fU1 U U U1 U U U U U
CPR24 [U] U U
CPR25 [U] U1 U
CPR26 U U U U U
CPR27 no
CPR28 no
CPR29 no
CPR30 bU U U {U} U U
CPR31 U U
CPR32 no
CPR33 no
CPR34 2LC [S] {S} S
CPR35 S S S
CPR36 [S] {S} S
CPR37 {S} no
CPR38 {U} no
CPR39 [S] S
CPR40/43/45/46 S S S
CPR41 S S S
CPR42 S S S
CPR44 S S S
CPR47 2LB {U} no
CPR48 S S S
CPR49 S S S
CPR50 S S S
CPR51 S S S
CPR52 S S S
CPR53 S S S
CPR54 S S S
CPR55 no
CPR56 no
CPR57 no
CPR58 fU fUmU1 US U1S US S US US US US U US
CPR59 bUS bUS US US US US US US US US US U US
CPR60 bS bS S S U1S S U1S US US S S US
CPR61 U U V&W U
CPR62 fU bU U U U U U {U} U U
CPR63 no
CPR64 U1 U
CPR65 2LC [S] {S} S
CPR66 S S S
CPR67 {U} no
CPR68 no
CPR69 fU1mU bU U U U U U U
CPR70 bUS fU1mUbS US US US US US US US US U US
CPR71 no
CPR72 2LC [S] S
CPR73 U U U
CPR74 [U] [U] U
CPR75 [U] U U
CPR76 U U
CPR77 U U U
CPR78 [fU] U U
CPR79 U1 U U {U} {U} U
CPR80 [fU] {U} U
CPR81 fU fU {U} U U
CPR82 3RA S US US U US
CPR83 mS1 S S S US U U US
CPR84/108 mU1 US US US U US
CPR85 no
CPR86 3RB S S S
CPR87 S S S
CPR88 S S S
CPR89 S S S
CPR90 S S S
CPR91 S S S
CPR92 3RC S S V&W V&W S
CPR93 S S S
CPR94 S S S
CPR95 S S S
CPR96 S S S
CPR97 S S S
CPR98 S US US {S} S
CPR99 3RC S S V&W V&W S
CPR100 S S S
CPR101 no
CPR102 no
CPR103 no
CPR104 no
CPR105 U1 U
CPR106 U U U1 U U U
CPR107 mS1 US S1 S S US S US
CPR109 S S S
CPR110 bU fUmU1 U U1 U U U U U U U U
CPR111 U U {U} U1 U
CPR112 no
CPR113 bU fU U U U {U} {U} U U
CPR114 U U U U U U
CPR115 2RB bS bS S S {S} S S US S US
CPR116 bU U U U U U U U U
CPR117/154 2RB bS bS S S S S S S S S
CPR118/119/121/158 bS bS S S S S S S S S
CPR120 fUbS bS US US S US US US US US
CPR122 fUmU1 bS1 S S US US US US US US
CPR123 fU1bS bS US US US US US US US US
CPR124 bU bU U U U U U U {U} U U
CPR125 bU bU U U U U U U U U U U
CPR126 bU bU U U U U U U U U U U
CPR127 bU bU U U U U U U U U U
CPR128 [mU] {U} {U} U U
CPR129 U U
CPR130 bU bU U U U U U U U U U U
CPR131 U U U U U U U
CPR132 U U U U
CPR133/153 [U] [U] U V&W U U
CPR134 [U] [U] [U] U
CPR135 bU bU U U U U U U U U U U U
CPR136 2LB S S S
CPR137 no
CPR138 U {U} U
CPR139 U U
CPR140 bU bU U U U U U U U U U U
CPR141 no
CPR142 3RC S S V&W V&W S
CPR143 [U] U U
CPR144 no
CPR145 2LC no
CPR146 fU1 U1 U U U U U U U1 U
CPR147 bU U {U} {U} U U
CPR148 3RB S S S
CPR149 S S S
CPR150 S S S {S} S
CPR151 U U U U U
CPR152 U U
CPR155 no
CPR156 3RB mU1 U
CPR157 fU U U {U} {U} U
CPR159 no
CPR160 bU bU U U U U U U
CPR161 [fUmU1] {U} U1 U
CPR162 bU bU U U U U U U U U
CPR163 U U U U U U
CPR164 fU fU U1 U
CPAP1-A U U [U1] U
CPAP1-B1 no
CPAP1-B2 [U] U1 U
CPAP1-C no
CPAP1-F U U {U} U
CPAP1-G U U U1 U U U U
CPAP1-H no
CPAP1-J no
CPAP1-K-PA [US] US
CPAP1-K-PB [S] S
CPAP1-M [mU] U U U U
CPAP1-N no
CPAP1-O mU U {U} U
CPAP3-A1a U U1 U U U U U U
CPAP3-A1b S US {S} US
CPAP3-A1c S US U {U} {U} US
CPAP3-B U U U U
CPAP3-C-PA-PC U U U1 U U U U U U
CPAP3-D1 U U U U U U U U
CPAP3-D2-PA-PB no
CPAP3-E U U U [U] {U} U
CPCFC1 fU U U U U U U
CPF1 US US US US S S S US
CPF2 fU mU US US US US US US U US
CPF3 fU1 bU U U U U U U U U
CPF4 bU bU U U U U U U U U
CPFL1 bU mU U U U U U U {U} U U
CPFL2 a [fS] U1S S U U US
CPFL3 b [fS] S S S S S
CPFL4/6 b [fS] S S S S S
CPFL5 b [fS] S S S S S
CPFL7 a [fS] S S S S S
CPLCA1 bS mUbS US US US US US S US {U} US US
CPLCA2 bS bUS US US US US S US S {U} S US
CPLCA3 bU U U U U U U U
CPLCG1 bU U U U U U U
CPLCG2 [U] U U
CPLCG3 [U] {S} {S} {S} U
CPLCG4 fU1 fU1mU U U U U U U U U
CPLCG5 bU bU U U U U U U U U
CPLCG6 A S S S S S S
CPLCG7 B S S S S S
CPLCG8 A S S S S S S
CPLCG9 A S S S S S S
CPLCG10 B S S S S S
CPLCG11 A S S S S S S
CPLCG12 B US US S S US
CPLCG13 A S S S S S S
CPLCG14 U U US US U {U} U U U
CPLCG15 mU1 fU1mU U U US U U U U US
CPLCG16 U U US US US
CPLCG17 A U1S S S S S US
CPLCG18 A S S S S S S
CPLCG19 A S S S S S S
CPLCG20 B S S S S S
CPLCG21 A US US S S S US
CPLCG22 U U U U U1 U
CPLCG23 no
CPLCG24 no
CPLCG25 A S S S S S S
CPLCG26 A US US S S {S} US
CPLCG27 A US S S S US
CPLCG28 S S [U1] U
CPLCG29 {U} no
CPLCP1 S S S S {U} S
CPLCP2 S US S1 US
CPLCP3 US US US US {U} S1 US
CPLCP4 no
CPLCP5 no
CPLCP6 S S S S S S S S S
CPLCP7 {U1S} {U1S} S {S} {S} US
CPLCP8 US S US US U US US U US
CPLCP9 US S US US S1 US
CPLCP10 bS US US S US US S S US US
CPLCP11 bUS US S U1S U US US US US US US
CPLCP12 fUS bUS US US US U US US US US US US
CPLCP13 C S S S S S
CPLCP14 C S S S S S
CPLCP15 C S S S S S
CPLCP16 C S S S S S
CPLCP17 C S S S S S
CPLCP18/19/23 C S S S S S
CPLCP20 C S S S S S
CPLCP21 C S S S S S
CPLCP22 C S S S S S
CPLCP24 C S S1 S S
CPLCP25 C US US S S US
CPLCP26 C U1S U1S S S S S S S1 US
CPLCP27 US US US
CPLCP28 no
CPLCW1 {US} {US} S {U} {S} US
CPLCW2/3 {S} {S} {S} {S} S
CPLCW4 {S} {S} {S} {S} S
CPLCW5 {S} {S} {S} {U} S
CPLCW6//8 S S S S
CPLCW7 {US} {US} {U} {S} US
CPLCW9 {S} {S} {S} {S} S
CPLCX1 {U1} U {U} U
CPLCX2 bU bU U U U U U U {U} U U U
CPLCX3 bU bU U U U U U U U U U U U
CPLCX4 bU U U U U
CPLCX5 fU U U U U U U U
CPLCX6 [no]
CPLCX7 [U] U
CPLCX8 [no]
CPLCX9 [U] {U} U
CPLCX10 bU bU U1 U U U U U U {U} U U
CPLCX11 U U
CPLCX12 [no]
CPLCX13 [U] [U] U
CPLCX14 no
CPLCX15 no
CPLCX16 no
CPLCX17 no
CPLCX18 no
CPLCX19 U U1 U1 U U U U U
CPLCX20 U U U U U
CPLCX21 no
CPLCX22 [bU] U
TWDL1 fU U U U U U U U U U U U
TWDL2 [fU] US US US US
TWDL3 fS1 fS US US US US
TWDL4 fS fS S S S S
TWDL5 US US U1S US
TWDL6 S S S S
TWDL7 US US US US
TWDL8 {S} {S} {S} no
TWDL9 bU fU1 U U U U U U {U} U U U
TWDL10 {S} S1 S
TWDL11 bU U U U U U US US US US US
TWDL12 fU1 U U U U U U U {S} U U
CPTC1 U U U
CPTC2 U U U
CPTC3 U U U
CPTC4 U U U
CPRC1 fU1 fU1 U1 [U] U
CPRC2 U1 U
CPRC3 U U U
CPRC4 mU U U U U U
CPX1 fU U U U U U U U U U1 U
CPX2 U U U U U U
yellow fU bU1 U U U U U U U U U U
yellow-e fUmU1 U U U U U U U U U U
yellow-f [fU] U U U U U
yellow-h U U U U U U U U
SUMMARY 19 no
35 no
24 NEW

U - unique peptide found in protein; U1 - only one peptide detected in only one sample.

S - peptide shared among proteins. S (bolded) - at least one peptide not found in a protein that had a unique peptide.

“m”, “f’ and “b” indicate source (male, female, both) of peptides found in proboscises or palps.

CPR protein groups are designated with color type in names: RR-1, green; RR-2, brown; unclassified, blue. Those scoring below cut-off score in CutProt-FamPred are in italics. Gray highlighting in “Protein Name” column indicates that peptides from that protein were identified in all six adult structures.

Colored highlighting indicates that the protein had not been identified in any other structure or was restricted to larvae. Square brackets [ ] in colored box indicate that VectorBase or Mastrobuoni et al., 2013 had peptides for this protein in other stages/structures.

Curly brackets { } surrounding U or S in boxes for antennae, legs or wings indicate that Masson et al., 2018 found peptide(s) for this structure when we had not. These brackets in larval columns indicate that the protein was not restricted to larvae based on Masson et al.

*

Antenna includes Johnston’s organ (JO). They were analyzed separately in Zhou et al. 2016 and JO not analyzed in Masson et al., 2018.

Data for whole adults from Zhou et al., 2017 S6_Table.

Gray highlighting in the” Up-to-date Summary” column indicates the 24 proteins identified by us for the first time in this study.

Gray highlighting in cells in the “whole adults” column indicates that the protein was identified only in adults in this and other studies.

Bolded “no” in Up-to-date summary column indicates that protein had no peptides in Mastrobuoni et al., 2013 or Masson et al., 2018 and/or on VectorBase as of5/16/18.

V&W indicates that even though no peptides were detected, antibodies raised against a peptide in that protein or sequence cluster were found in that structure by EM immunolocalization (Vannini and Willis, 2016).

A no in square brackets, means that trypsin peptides were unlikely. See Supplementary File 3.

AGAP numbers and complete amino acid sequences are in Supplementary File 3.