. Author manuscript; available in PMC: 2019 Sep 1.

Published in final edited form as: Proteomics Clin Appl. 2018 Apr 18;12(5):e1700182. doi: 10.1002/prca.201700182

TABLE 1.

Spore coat/exosporium and immunoreactive proteins of epidemic C. difficile R20291 strain identified in a 2-DE gel system

Spot #	Gene name^a	Uniprot ID^a	Protein Description^a	Score^b	MW (Da)^c	pI^d	Peptides^e	% coverage^f
1	CDR20291_1511	C9YLQ8	Uncharacterized protein (CotA)	453	35209	3.98	8	17.2
2	CDR20291_1282	C9YL30	Peroxiredoxin (CotE)	838	81612	5.25	40	33.9
3	CDR20291_0654	C9YJA5	Putative carbon monoxide dehydrogenase/acetyl-CoA synthase complex, methyltransferase subunit	288	29762	4.78	25	71.3
4	CDR20291_0926	C9YK25	Uncharacterized protein (CdeC)	330	46958	4.84	27	40
5	CDR20291_2291	C9YNX8	Putative spore-coat protein (CotCB)	249	21497	4.68	19	72.1

Functional annotations were retrieved from Swiss Protein^a database (www.uniprot.org )

The threshold was set up by the server at the significance level P≤0.05 for random hit; individual ion scores greater than 17 were taken as significant match for identity or extensive homology, based in Mowse score (http://www.matrixscience.com )

The molecular weight of the protein identified from the database.

pI: isoelectric point of the protein identified.

The number of matching peptides to the target protein identified

The portion of the protein sequence that was observed by MS/MS analysis of the peptide mapping