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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Chromosome Res. 2018 Aug 24;26(4):277–295. doi: 10.1007/s10577-018-9584-1

Figure 1. Combination of micromechanics measurement of mitotic chromosomes with RNAi depletion of condensin.

Figure 1.

(A) Schematic diagram of experimental micropipette setup for chromosome micromanipulation. One of the two pipettes (force pipette) has a long taper with a spring constant of 30–200 pN/μm, which is used to measure forces in the range of 10–1000 pN by monitoring its bending. A third pipette is used as the spray pipette to spray reagents directly onto an isolated chromosome. Lower right insert shows a mitotic chromosome from human HeLa cells, and the lower left insert shows its linear, reversible elasticity. (B-C) Depletion of SMC2 (hCAP-E) using siRNA in HeLa cells. Protein knockdown is verified by western blot (B), and (C) immunofluorescence staining of SMC2 in fixed cells 36 hrs after siRNA treatment, using anti-SMC2 antibody in both cases. Bar = 5 μm.